FIGURE

Fig. 2

ID
ZDB-FIG-170407-2
Publication
Zelina et al., 2014 - Signaling switch of the axon guidance receptor Robo3 during vertebrate evolution
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Fig. 2

Mammalian Robo3 Does Not Bind Slits with High Affinity

(A) hSlit2-D2-AP binds to COS cells expressing mammalian rRobo1A and rRobo2B but not to cells expressing mammalian Robo3A.1 or Robo3B.2.

(B) Slit binding is lost in cells expressing rRobo1N88P/K90R/L130P or rRobo1L130P but restored in cells expressing mRobo3P84N/R86K/P126L or mRobo3P126L.

(C) zSlit2-D2-AP binds to COS cells expressing zRobo2 or zRobo3A.1 but not to cells expressing zRobo3N83P/K85R/L125P or zRobo3L125P.

(D) xSlit2-D2-AP binds to COS cells expressing xRobo3A.1 but not xRobo3N85P/K87R/L127P.

(E) Scatchard analysis of Slit2 binding affinity to Robo receptors. The data shown are representative of at least three independent experiments.

See also Figure S2.
Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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