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Fig. 2

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ZDB-IMAGE-170413-47
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Figures for Zelina et al., 2014
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Figure Caption

Fig. 2

Mammalian Robo3 Does Not Bind Slits with High Affinity

(A) hSlit2-D2-AP binds to COS cells expressing mammalian rRobo1A and rRobo2B but not to cells expressing mammalian Robo3A.1 or Robo3B.2.

(B) Slit binding is lost in cells expressing rRobo1N88P/K90R/L130P or rRobo1L130P but restored in cells expressing mRobo3P84N/R86K/P126L or mRobo3P126L.

(C) zSlit2-D2-AP binds to COS cells expressing zRobo2 or zRobo3A.1 but not to cells expressing zRobo3N83P/K85R/L125P or zRobo3L125P.

(D) xSlit2-D2-AP binds to COS cells expressing xRobo3A.1 but not xRobo3N85P/K87R/L127P.

(E) Scatchard analysis of Slit2 binding affinity to Robo receptors. The data shown are representative of at least three independent experiments.

See also Figure S2.

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