Fig. 4 S1

Temporal pattern of hair cell differentiation in AM and PM.

Tg[Brn3c:GFP] embryos injected with H2B-mCherry mRNA at 1cell-stage (with/without MO-neurog1) were imaged from 24 hpf to 42 hpf. (a) Graphs showing the increase of differentiated hair cells in the anterior (AM) and posterior (PM) maculae over time; the final number of differentiated hair cells at 42 hpf is indicated. Each line corresponds to a differentiated hair cell plotted from and color-coded for time of differentiation (see legend). Tether cells are depicted as white lines. Note the temporal differences in the development of the PM between control and MO-neurog1 embryos, with no major changes in the spatial pattern (Figure 4). Asterisk depicts an apoptotic hair cell. ID Datasets: 140507aX for control, 140519aX for MO-neurog1. (b) Spatiotemporal pattern of hair cell differentiation of the AM and PM of both ears of Tg[Brn3c:GFP]Tg[Isl3:GFP] embryo injected with lyn-TdTomato and H2B-mCherry mRNA at 1cell-stage (imaged from 25 hpf to 36 hpf). Differentiated hair cells were tracked and reconstructed colored cell centers overlaid with the corresponding raw images (hair cell fate/SAG in grey level as 3D-rendering). Graphs showing the increase of differentiated hair cells in the AM and PM over time; the final number of differentiated hair cells at 36 hpf is indicated. ID Dataset: 140402aX. (c) Spatiotemporal pattern of hair cell differentiation of AM and PM. Tg[Brn3c:GFP] embryo injected with lyn-TdTomato mRNA at 1cell-stage and imaged from 25 hpf to 45 hpf. Graphs displaying the increase of differentiated hair cells in AM/PM. Hair cell progenitors and differentiated hair cells were tracked (except for the one encircled in blue), and reconstructed colored cell centers overlaid with the corresponding raw images (MIP of lynTomato and GFP signal of a few slices in grey level). ID Dataset: 130326aX. Legend in panel (a) applies to all plots and diagrams of the figure.