Fig. 3

Ergosterol Enhances Murine Hematopoietic Cell Homing

(A) Structure of ergosterol, a member of the sterol family of compounds.

(B) BLI at 1 dpt of fish receiving ergosterol-treated cells in the secondary screen. The region of Interest (ROI) and donor cell radiance values of the WKM are shown. Some residual signal can be seen at the injection site. Right panel shows difference in radiance between vehicle and ergosterol-treated homed donor cells. Data shown are mean ± SD from five individual recipients.

(C) Flow cytometry gating for the identification of Lin^?SCA-1⁺KIT ⁺ donor cells after murine HCT. Initial gates were used to identify mononuclear cells that were propidium iodide (PI) negative.

(D) Ten million CD45.1 donor cells in 50 ?M ergosterol (circulating concentration estimated at 10 ?M) or equal v/v vehicle were transplanted into CD45.2 lethally irradiated recipients. Bilateral femur marrow was harvested from recipients 16 hr post-transplant. Flow cytometry was used to quantify absolute numbers of homed donor cells using the gating strategy in (C). Data shown are mean ± SD and p values from a Student's t test (n = 10?11 animals/group in two pooled experiments).