FIGURE

Fig. 1

ID
ZDB-FIG-170222-19
Publication
Ben-Moshe Livne et al., 2016 - Genetically Blocking the Zebrafish Pineal Clock Affects Circadian Behavior
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Fig. 1

Generation of Tg(aanat2:EGFP-?CLK) fish.

(A) Schematic representation of the transgenic construct used for the generation of Tg(aanat2:EGFP-?CLK) fish. The pT2-aanat2:EGFP-2A-?clocka-5×MYC plasmid consists of two arms from the Tol2 transposable element (black), aanat2 regulatory regions (gray), EGFP coding sequence (CDS; green), 2A peptide sequence (orange), ?CLK CDS (blue), 5×Myc tags (purple) and SV40 poly(A) signal (yellow). (B and B') EGFP expression is restricted to the pineal gland of Tg(aanat2:EGFP-?CLK) larvae. Dorsal views of the head region of 7-day post-fertilization (dpf) larvae, anterior to the top; confocal z-stack projection (B) and a single confocal plane (B'). (C) Immunostaining with anti-Myc antibody confirms that ?CLK is specifically expressed in the pineal gland of Tg(aanat2:EGFP-?CLK) larvae. Dorsal view of the head region of a 5-dpf larva, anterior to the top; confocal z-stack projection.
Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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