ZFIN Image: Ben-Moshe Livne et al., 2016, Fig. 1

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Figure Caption

Fig. 1

Generation of Tg(aanat2:EGFP-ΔCLK) fish.

(A) Schematic representation of the transgenic construct used for the generation of Tg(aanat2:EGFP-ΔCLK) fish. The pT2-aanat2:EGFP-2A-Δclocka-5×MYC plasmid consists of two arms from the Tol2 transposable element (black), aanat2 regulatory regions (gray), EGFP coding sequence (CDS; green), 2A peptide sequence (orange), ΔCLK CDS (blue), 5×Myc tags (purple) and SV40 poly(A) signal (yellow). (B and B') EGFP expression is restricted to the pineal gland of Tg(aanat2:EGFP-ΔCLK) larvae. Dorsal views of the head region of 7-day post-fertilization (dpf) larvae, anterior to the top; confocal z-stack projection (B) and a single confocal plane (B'). (C) Immunostaining with anti-Myc antibody confirms that ΔCLK is specifically expressed in the pineal gland of Tg(aanat2:EGFP-ΔCLK) larvae. Dorsal view of the head region of a 5-dpf larva, anterior to the top; confocal z-stack projection.

Figure Data

Construct: Tg(aanat2:EGFP-2A-Dclocka-5xMYC)

Acknowledgments

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