FIGURE

Fig. S7

ID

ZDB-FIG-170220-44

Publication

Wild et al., 2017 - Neuronal sFlt1 and Vegfaa determine venous sprouting and spinal cord vascularization

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Fig. S7

Spinal cord vascularization in WT zebrafish

(a,b) Neuronal cells were isolated from Tg(Xla.Tubb:DsRed)^zf148 embryos at 3dpf by FACS with indicated gating settings. About 12% of all intact cells were DsRed+ neurons prior to sorting (Pre-sort). (c,d) Post-sorting analysis showed that sorted neuronal cells are enriched to 51% neuronal DsRed+ cells. DsRed- cells contained less than 1.7 % DsRed+ cells. (e) Percentage of aISV and vISV giving rise to sprouts at level of neural tube in late stage WT embryo; 400 ISVs in n=20 embryos. (f) Nuclear positioning in sprout contributing to spinal cord vascularization in wildtype Tg(kdrl:has.HRAS-mcherry)^s916;Tg(fli1a:nEGFP)^y7 at 12dpf; representative image from 6 embryos (g) Quantitative PCR for sflt1 at indicated time points. Note decreased expression of sflt1 associates with sprout appearance and spinal cord vascularization. mean ± s.e.m, n=3 experiments, 30 embryos/experiments dpf, days post fertilization; ISV, intersegmental vessel (a – artery, v – vein). Scale bar, 10μm in f.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.