FIGURE

Fig. S4

ID

ZDB-FIG-170209-22

Publication

Sotolongo-Lopez et al., 2016 - Genetic Dissection of Dual Roles for the Transcription Factor six7 in Photoreceptor Development and Patterning in Zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. S4

Levels of mitosis are altered in six7^fl4 at 56 hpf.

(A) Confocal immunofluorescent images labeled for rods (4C12, red) from WT, six7^fl4 and six7^fl4/p23ahub retinas at 4 dpf. (B) Retinal cryosections from carrier animals (six7^fl4/+, n = 5, 1–2 sections/retina) and six7 (n = 11) embryos at 56 hpf co-labeled for TUNEL (red) and PH3 (green), nuclei counterstained with DAPI. No differences in TUNEL labeling were detected. Graphs showing the number of PH3⁺ cells by section (excluding CMZ). Number of PH3⁺ cells is significantly greater in ONL of six7^fl4 mutants at 56 hpf. Un-paired Student t test with Welch’s correction, *p<0.05). (C) Retinal cryosections from WT and six7^fl4 embryos at 4 dpf immunolabeled for rods (4C12, red) and the green-, UV-, blue- and red-sensitive opsins (green). Nuclei were counterstained with DAPI (blue). Dorsal is up. Depleted green-sensitive opsin expression is noticeable in six7^fl4, but other opsins appear unaltered.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.