Fig. 1

adgra2 and ouchless mutations fail to complement. (A) Dorsal views and (A′) quantification of EGFP⁺ DRG neurons in 72 hpf Tg(neurog1:EGFP) larvae resulting from crossing ouchless and adgra2 alleles. Red arrowheads indicate DRG neurons. Center and right columns illustrate the corresponding adult pigmentation patterns. The neurog1:EGFP⁺ DRG were counted on one side of the 72 hpf larvae. Brackets indicate discontinuous dorsal melanophore stripes. (B) Confocal z-stack projections showing the brain vasculature of Tg(kdrl:GFP) WT and mutant embryos at 60 hpf in dorsal views (top) and 3D wire diagram representation of the cranial vessels, with intracerebral vessels (central arteries or CtAs) in red and perineural vessels in white (bottom). (B′) Quantification of the corresponding hindbrain CtAs. (C) Confocal projections in dorsal views of WT and mutant Tg(7xTCF-Xia.Siam:GFP); Tg(kdrl:ras-mCherry) embryos at 32 hpf. Boxes define the areas magnified in the bottom row. (D) Confocal projections of the ouchless mutant Tg(neurog1:EGFP) DRG at 72 hpf (top panels) and Tg(kdrl:GFP) cranial vasculature at 60 hpf (bottom panels) injected, or not, with 100 pg of adgra2 mRNA at the one-cell stage. Red arrowheads indicate DRG neurons. (E) Quantification of neurog1:EGFP⁺ DRG at 72 hpf and (F) hindbrain CtAs at 60 hpf in WT and ouchless mutant animals injected, or not, with 100 pg of adgra2 mRNA at the one-cell stage. The neurog1:EGFP⁺ DRG were counted on one side of the 72 hpf larvae. Error bars represent median±interquartile range; *P<0.05, ***P<0.001 (Kruskal–Wallis test). PHBC, primordial hindbrain channel. Scale bars: 50 µm.