FIGURE

Fig. 4

ID

ZDB-FIG-170203-14

Publication

Miao et al., 2017 - Translation repression by maternal RNA binding protein zar1 is essential for early oogenesis in zebrafish

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Fig. 4

Zar1 deficiency causing all-male phenotype is due to p53-mediated apoptosis. (A-C) TUNEL staining of ovary sections of zar1 homozygotes and control siblings at 37-40?dpf. Obvious apoptotic cells are observed in immature ovaries in zar1 homozygotes (A), but not in sibling controls (B). Quantification of apoptotic cells is shown in C; 18 sections from 6 juveniles were counted for each genotype. (D) Gender analysis of zar1^?/? homozygotes on different p53 genotype backgrounds (p53^+/+, p53^+/? and p53^?/?). Females are only observed in p53^?/?;zar1^?/? double homozygous mutants. (E,F) H&E staining of sections of p53^?/?;zar1^+/? and p53^?/?;zar1^?/? adult ovaries. p53^?/?;zar1^?/? ovaries are morphologically normal. (G-J) TUNEL staining of ovary sections of p53^?/?;zar1^?/? ovaries at 37-40?dpf with p53^?/?;zar1^+/? and p53^+/?;zar1^?/? ovaries as controls. (J) Quantification of apoptotic cells. Nine sections from three juveniles were counted for each genotype. Scale bar: 20??m.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zar1^gd5/gd5 (TU) tp53^zdf1/+; zar1^gd5/gd5 tp53^zdf1/zdf1; zar1^gd5/gd5
Observed In:	female organism female sex determination oocyte growth ovary apoptotic process
Stage Range:	Days 30-44 to Adult

Phenotype Detail

Acknowledgments

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