FIGURE

Fig. 4

ID

ZDB-FIG-170201-47

Publication

Eisenhoffer et al., 2017 - A toolbox to study epidermal cell types in zebrafish

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Fig. 4

Crossing epithelial Gal4 lines to UAS lines that provide tools for developmental studies. (A,B) Cells can be fluorescently labeled and tracked over time with the periderm Gal4 line (zc1044A) driving expression of photo-convertible protein Kaede, Tg(UAS-E1b:Kaede)^s1999t. Expression of Kaede is detected as green fluorescence before exposure to blue (UV) light (A), and red (shown here as magenta) after exposure (B). (C-F) The labeled epithelial cells can be readily ablated using the nitroreductase–metronidazole system, Tg(UAS-E1b:nfsB-mCherry). (C-D) Representative bright-field images of wild-type (C) and zc1036A (D) 48 hpf embryos after 24 h treatment with MTZ to ablate the cells. Arrowhead in D′ denotes defects in the developing median fin epidermis. (E-F) The MTZ-exposed zc1036A cells undergo apoptosis (activated caspase-3-positive cells in green) and cause defects in the developing embryo. C-D represent data from a single experiment performed in triplicate. Scale bars: 25 μm in A-B, 250 μm in C-D, 50 μm in E-F.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.