ZFIN ID: ZDB-PUB-160507-10
A toolbox to study epidermal cell types in zebrafish
Eisenhoffer, G.T., Slattum, G., Ruiz, O.E., Otsuna, H., Bryan, C.D., Lopez, J., Wagner, D.S., Bonkowsky, J.L., Chien, C.B., Dorsky, R.I., Rosenblatt, J.
Date: 2017
Source: Journal of Cell Science   130(1): 269-277 (Journal)
Registered Authors: Bonkowsky, Joshua, Chien, Chi-Bin, Dorsky, Richard, Eisenhoffer, George, Otsuna, Hideo, Wagner, Daniel
Keywords: Epithelia, zebrafish, In vivo
MeSH Terms:
  • Animals
  • Cell Death/drug effects
  • Cell Division/drug effects
  • Crosses, Genetic
  • Cytological Techniques/methods*
  • DNA-Binding Proteins/metabolism
  • Enhancer Elements, Genetic/genetics
  • Epidermis/cytology*
  • Epidermis/drug effects
  • Epidermis/ultrastructure
  • Epithelial Cells/cytology
  • Epithelial Cells/drug effects
  • Female
  • Gene Expression Regulation, Developmental/drug effects
  • Imaging, Three-Dimensional
  • Male
  • Morpholinos/pharmacology
  • Time Factors
  • Transcription Factors/metabolism
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/metabolism
PubMed: 27149923 Full text @ J. Cell Sci.
FIGURES
ABSTRACT
Epithelia provide a critical protective barrier for our organs and are also the sites where most carcinomas form. Most studies on epithelia and carcinomas use cell culture or organisms where high-resolution live imaging is inaccessible without invasive techniques. Here, we introduce the developing zebrafish epidermis as an excellent in vivo model system for studying a living epithelium. We developed tools to fluorescently tag specific epithelial cell types and express genes in a mosaic fashion using five GAL4 lines identified from an enhancer trap screen. When crossed to a variety of UAS effector lines, we can now track, ablate or monitor single cells at sub-cellular resolution. Using photo-cleavable morpholino oligonucleotides that target GAL4, we can also express genes in a mosaic fashion at specific times during development. Together, this system provides an excellent in vivo alternative to tissue culture cells, without the intrinsic concerns of culture conditions or transformation, and enables the investigation of distinct cell types within living epithelial tissues.
ADDITIONAL INFORMATION