FIGURE

Fig. 3

ID

ZDB-FIG-170123-11

Publication

Miranda-Rodríguez et al., 2017 - RhoA/ROCK pathway activity is essential for the correct localization of the germ plasm mRNAs in zebrafish embryos

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Fig. 3

RhoA inhibition interferes with animal germ plasm localization. RhoA immunolocalization (A, B, G and H) vasa mRNA localization (C, and D), nanos mRNA localization (I and J) by fluorescent whole mount in situ hybridization. Embryos injected with buffer (A, C and E; n=9) and (G, I and K; n=8) or with the RhoA inhibitor C3 (B, D and F; n=4) and (H, J and L; n=15). (E and F; K and L) images superimpositions of double labeled embryos with RhoA immunolocalization (green) and vasa or nanos fluorescent in situ hybridization (red) respectively. Insets show image magnification of the corresponding first two-cleavage furrow intersection (e1, f1, k1 and l1) or cleavage furrow edge (e2, f2, k2 and l2). Scale bar 250 μm.

Expression Data

Genes:	ddx4 nanos3
Antibody:	Ab1-rhoa
Fish:	WT
Condition:	chemical treatment by injection: C3 exoenzyme
Anatomical Term:	blastodisc cleavage furrow
Stage:	4-cell

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Condition:	chemical treatment by injection: C3 exoenzyme
Observed In:	blastodisc cleavage furrow
Stage:	4-cell

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 421(1), Miranda-Rodríguez, J.R., Salas-Vidal, E., Lomelí, H., Zurita, M., Schnabel, D., RhoA/ROCK pathway activity is essential for the correct localization of the germ plasm mRNAs in zebrafish embryos, 27-42, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.