ZFIN ID: ZDB-FIG-161221-18
Yu et al., 2016 - Eyes shut homolog is required for maintaining the ciliary pocket and survival of photoreceptors in zebrafish. Biology Open   5(11):1662-1673 Full text @ Biol. Open
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Term:
Stage Range: Days 30-44 to Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 5

Reduction of GNAT2 protein and cone density in EYS-deficient zebrafish. Western blotting for GNAT2 was performed on retinal lysates from 2 mpf and 9 mpf zebrafish. Cone density was determined by counting 1D4-, green opsin-, blue opsin-, and UV opsin-positive cells in the dorsal and ventral retina. (A) Western blot for GNAT2 and α-tubulin. Note that while GNAT2 signal was not changed in 2 mpf mutant zebrafish, it was diminished in 9 mpf mutant zebrafish. (B) Quantification of western blot shown in A. *P<0.05, Student's t-test, two-tailed. Error bars indicate SEM (standard error of the mean). (C) Cone density in EYS-deficient zebrafish was normal at 40 dpf but reduced at 6 and 8 mpf. *P<0.05; **P<0.01 (repeated measures ANOVA). Error bars indicate s.e.m.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
opn1mw1 WT control Days 30-44 retina RTPCR
Adult retina RTPCR
eyssny10/sny10 standard conditions Days 30-44 retina RTPCR
Adult retina RTPCR
eyssny14/sny14 standard conditions Days 30-44 retina RTPCR
Adult retina RTPCR
opn1sw1 WT control Days 30-44 retina RTPCR
Adult retina RTPCR
eyssny10/sny10 standard conditions Days 30-44 retina RTPCR
Adult retina RTPCR
eyssny14/sny14 standard conditions Days 30-44 retina RTPCR
Adult retina RTPCR
opn1sw2 WT control Days 30-44 retina RTPCR
Adult retina RTPCR
eyssny10/sny10 standard conditions Days 30-44 retina RTPCR
Adult retina RTPCR
eyssny14/sny14 standard conditions Days 30-44 retina RTPCR
Adult retina RTPCR
rho WT control Days 30-44 retina RTPCR
Adult retina RTPCR
eyssny10/sny10 standard conditions Days 30-44 retina RTPCR
Adult retina RTPCR
eyssny14/sny14 standard conditions Days 30-44 retina RTPCR
Adult retina RTPCR
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-gnat2 WB WT control Days 45-89 retina
WB Adult retina
WB eyssny10/sny10 standard conditions Days 45-89 retina
WB Adult retina
WB eyssny14/sny14 standard conditions Days 45-89 retina
WB Adult retina
Phenotype Details
Fish Conditions Stage Phenotype
eyssny10/sny10 standard conditions Adult retina opn1mw1 expression decreased amount, abnormal
Adult retina opn1sw2 expression decreased amount, abnormal
Adult retina rho expression decreased amount, abnormal
Adult retina opn1sw1 expression decreased amount, abnormal
Adult retina ab2-gnat2 labeling decreased amount, abnormal
eyssny14/sny14 standard conditions Adult retina rho expression decreased amount, abnormal
Adult retina opn1mw1 expression decreased amount, abnormal
Adult retina opn1sw1 expression decreased amount, abnormal
Adult retina ab2-gnat2 labeling decreased amount, abnormal
Adult retina opn1sw2 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Biology Open for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Biol. Open