ZFIN ID: ZDB-FIG-161018-8
Lu et al., 2016 - IGFBP1 increases β-cell regeneration by promoting α- to β-cell transdifferentiation. The EMBO journal   35(18):2026-44 Full text @ EMBO J.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Term:
Stage: Days 30-44
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Days 30-44

Fig. 6

Igfbp1a also increases βcell regeneration in 1 month old zebrafish

A, B. Representative confocal projections of the whole pancreas (dashed lines) of 35dayold Tg(ins:H2BGFP);Tg(ins:FlagNTR) transgenics, with or without Tg(bactin:igfbp1a), that were subjected to βcell ablation between day 30 and 31 and then allowed to regenerate for 4 days. Scale bars represent 100 µm.

C. Body length of control and Tg(bactin:igfbp1a) zebrafish; ****< 0.0001.

D-H. Quantification of βcell regeneration was automated with an ImageJ script. (D) Insulinpositive area per zebrafish. (E) Relative insulinpositive area per body length; **< 0.01. (F) The percentage of the pancreas area that was insulinpositive was significantly larger in Tg(bactin:igfbp1a) than in controls; ****< 0.0001. (G) The total number of recorded units of adjacent ins:H2BGFP+ pixels (units ranging from single β cells to βcell clusters) did not differ between control and Tg(bactin:igfbp1a) zebrafish. (H) The size of recorded ins:H2BGFP+ units was on average larger in Tg(bactin:igfbp1a) than in controls; *= 0.039. = 26 in the control group, = 27 in the Tg(bactin:igfbp1a) group.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP s950Tg ; ki103Tg ; s960Tg chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole Days 30-44 regenerating tissue pancreatic B cell IHC
s960Tg ; s950Tg chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole Days 30-44 regenerating tissue pancreatic B cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s950Tg ; ki103Tg ; s960Tg chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole Days 30-44 pancreatic B cell regeneration increased occurrence, abnormal
Days 30-44 whole organism decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal The EMBO journal for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ EMBO J.