FIGURE

Fig. 6

ID

ZDB-FIG-160923-5

Publication

Qiu et al., 2016 - Embryonic hematopoiesis in vertebrate somites gives rise to definitive hematopoietic stem cells

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Fig. 6

Analysis of sHPSCs in larvae and adults. (A) Co-colonization of foxc1⁺ sHPSCs (indicated by arrows) with cmyb⁺ HSCs in the kidney of a Tg(foxc1b:EOS;cmyb:gfp) transgenic larva, which was photoactivated in five pairs of posterior somites at the 25s stage. gb, gall bladder with red autofluorescence. (B) Detection of sPHSCs by flow cytometry in adult (3–6 months old) kidney marrow of various transgenic lines. The representative result was shown for one fish, and the average ratio (Av.) of EOS⁺ or GFP⁺ sHPSCs from three fish was indicated in parenthesis. (C) EOS⁺ or GFP⁺ sHPSCs sorted from the adult kidney marrow of a representative fish in various transgenic lines were re-analyzed by forward and side scatter. Different scatter fractions were circled with indicated percentage of cells. The yellowed area represented the sorting result of the WKM. The average percentage from three analyzed fish was written in red. (D) Expression levels of a set of muscle/skeletal or hematopoietic marker genes in twist1a⁺ or fmyhc2⁺ sHPSCs were analyzed by qRT-PCR compared to cmyb⁺ hematopoietic cells. GFP⁺ cells were sorted from the kidney marrow of corresponding transgenic adult fish. Data were shown as means from three experiments with the error bar indicated. *P < 0.05; **P < 0.01.

Expression Data

Genes:	EGFP Eos
Fish:	tsu2013Tg; zf169Tg
Anatomical Term:	head kidney hematopoietic multipotent progenitor cell
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Mol. Cell Biol.