PUBLICATION

Embryonic hematopoiesis in vertebrate somites gives rise to definitive hematopoietic stem cells

Authors
Qiu, J., Fan, X., Wang, Y., Jin, H., Song, Y., Han, Y., Huang, S., Meng, Y., Tang, F., Meng, A.
ID
ZDB-PUB-160603-3
Date
2016
Source
Journal of molecular cell biology   8(4): 288-301 (Journal)
Registered Authors
Meng, Anming
Keywords
blood, definitive hematopoiesis, embryos, primitive hematopoiesis, somite, stem cells, zebrafish
MeSH Terms
  • Gene Expression Profiling
  • Hematopoietic Stem Cells/cytology*
  • Hematopoietic Stem Cells/metabolism
  • Hematopoietic Stem Cells/radiation effects
  • Gene Expression Regulation, Developmental/radiation effects
  • Neovascularization, Physiologic/genetics
  • Neovascularization, Physiologic/radiation effects
  • Cell Differentiation/genetics
  • Cell Differentiation/radiation effects
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Somites/cytology*
  • Somites/embryology*
  • Somites/radiation effects
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/radiation effects
  • Aging
  • Mesoderm/cytology
  • Green Fluorescent Proteins/metabolism
  • Blood Cells/metabolism
  • Animals
  • Hematopoiesis*/radiation effects
  • Animals, Genetically Modified
  • Light
(all 24)
PubMed
27252540 Full text @ J. Mol. Cell Biol.
Abstract
Hematopoietic stem cells (HSCs) replenish all types of blood cells. It is debating whether HSCs in adults solely originate from the aorta-gonad-mesonephros (AGM) region, more specifically, the dorsal aorta, during embryogenesis. Here we report that somite hematopoiesis, a previously unwitnessed hematopoiesis, can generate definitive HSCs (dHSCs) in zebrafish. By transgenic lineage tracing, we found that a subset of cells within the forming somites emigrate ventromedially and mix with lateral plate mesoderm-derived primitive hematopoietic cells before the blood circulation starts. These somite-derived hematopoietic precursors and stem cells (sHPSCs) subsequently enter the circulation and colonize the kidney of larvae and adults. RNA-seq analysis reveals that sHPSCs express hematopoietic genes with sustained expression of many muscle/skeletal genes. Embryonic sHPSCs transplanted into wild-type embryos expand during growth and survive for life time with differentiation into various hematopoietic lineages, indicating self-renewal and multipotency features. Therefore, the embryonic origin of dHSCs in adults is not restricted to the AGM.
Genes / Markers
Figures
Figure Gallery (15 images) / 2
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
m39
    Deficiency
    s843TgTransgenic Insertion
      sd2TgTransgenic Insertion
        tsu054GtTransgenic Insertion
        tsu122TgTransgenic Insertion
          tsu2012TgTransgenic Insertion
            tsu2013TgTransgenic Insertion
              tsu20142TgTransgenic Insertion
                y1TgTransgenic Insertion
                  zf169TgTransgenic Insertion
                    1 - 10 of 11
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                    Human Disease / Model
                    No data available
                    Sequence Targeting Reagents
                    Target Reagent Reagent Type
                    cdx4MO1-cdx4MRPHLNO
                    etsrpMO2-etsrpMRPHLNO
                    kdrlMO1-kdrlMRPHLNO
                    trim33MO3-trim33MRPHLNO
                    1 - 4 of 4
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                    Fish
                    Antibodies
                    No data available
                    Orthology
                    No data available
                    Engineered Foreign Genes
                    Marker Marker Type Name
                    DsRedEFGDsRed
                    EGFPEFGEGFP
                    EosEFGEos
                    GFPEFGGFP
                    1 - 4 of 4
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                    Mapping
                    No data available