ZFIN ID: ZDB-PUB-160603-3
Embryonic hematopoiesis in vertebrate somites gives rise to definitive hematopoietic stem cells
Qiu, J., Fan, X., Wang, Y., Jin, H., Song, Y., Han, Y., Huang, S., Meng, Y., Tang, F., Meng, A.
Date: 2016
Source: Journal of molecular cell biology   8(4): 288-301 (Journal)
Registered Authors: Meng, Anming
Keywords: blood, definitive hematopoiesis, embryos, primitive hematopoiesis, somite, stem cells, zebrafish
MeSH Terms:
  • Aging
  • Animals
  • Animals, Genetically Modified
  • Blood Cells/metabolism
  • Cell Differentiation/genetics
  • Cell Differentiation/radiation effects
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/radiation effects
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental/radiation effects
  • Green Fluorescent Proteins/metabolism
  • Hematopoiesis*/radiation effects
  • Hematopoietic Stem Cells/cytology*
  • Hematopoietic Stem Cells/metabolism
  • Hematopoietic Stem Cells/radiation effects
  • Light
  • Mesoderm/cytology
  • Neovascularization, Physiologic/genetics
  • Neovascularization, Physiologic/radiation effects
  • Somites/cytology*
  • Somites/embryology*
  • Somites/radiation effects
  • Zebrafish/embryology*
  • Zebrafish/genetics
PubMed: 27252540 Full text @ J. Mol. Cell Biol.
Hematopoietic stem cells (HSCs) replenish all types of blood cells. It is debating whether HSCs in adults solely originate from the aorta-gonad-mesonephros (AGM) region, more specifically, the dorsal aorta, during embryogenesis. Here we report that somite hematopoiesis, a previously unwitnessed hematopoiesis, can generate definitive HSCs (dHSCs) in zebrafish. By transgenic lineage tracing, we found that a subset of cells within the forming somites emigrate ventromedially and mix with lateral plate mesoderm-derived primitive hematopoietic cells before the blood circulation starts. These somite-derived hematopoietic precursors and stem cells (sHPSCs) subsequently enter the circulation and colonize the kidney of larvae and adults. RNA-seq analysis reveals that sHPSCs express hematopoietic genes with sustained expression of many muscle/skeletal genes. Embryonic sHPSCs transplanted into wild-type embryos expand during growth and survive for life time with differentiation into various hematopoietic lineages, indicating self-renewal and multipotency features. Therefore, the embryonic origin of dHSCs in adults is not restricted to the AGM.