FIGURE

Fig. 2

ID

ZDB-FIG-160923-2

Publication

Qiu et al., 2016 - Embryonic hematopoiesis in vertebrate somites gives rise to definitive hematopoietic stem cells

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Fig. 2

Photoconverted hematopoietic cells are derived from somites but not LPM-derived blood precursors. (A-D) Dorsal somitic cells have a certain hematogenic activity at the 15s stage. The dorsal portion (above the notochord) of all somites in Tg(foxc1b:EOS) embryos was irradiated to avoid flashing the LPM-derived hematopoietic precursors. The posterior trunk region was shown (A) with the boxed area enlarged (B) and an optical cross section shown in C. The blood cells taken from 10 irradiated embryos in group at 30 hpf were analyzed by flow cytometry for red-EOS⁺ cells (D). (E-M) A downward irradiated single somite generates hematopoietic cells. The eighth somite on one side of a Tg(foxc1b:EOS;fli1a:gfp) embryo at the 20s stage was irradiated from the dorsal downward and the embryo was observed laterally (anterior to the left) by confocal microscopy at the 28s stage. (E-G) Trunk region focusing on the notochord. (H-J) An optical cross section from (E to G) with a photoconverted blood cell (indicated by an arrow) within the vessel. (K-M) An enlarged ICM region. Photoconverted blood cells were indicated by arrows.

Expression Data

Genes:	EGFP Eos
Fish:	tsu2013Tg; y1Tg
Anatomical Terms:	blood cell blood island blood cell hematopoietic cell somite trunk vasculature
Stage Range:	14-19 somites to 26+ somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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