Fig. 5

Effect of Zip10 knockdown on expression of genes involved in EMT during gastrulation of the zebrafish embryo 10 hpf

(A) ISH of E-cadherin (cdh1) mRNA in zebrafish wild-type embryos (WT), control embryos injected with a scrambled morpholino (Ctrl^MO), and in embryos injected at 1-4 cell stage with a translation blocking morpholino against zip10 (Zip10^MO) at 10 hpf. Ten embryos from each group were assessed and the experiment was repeated with the same results. (B) Expression of cdh1 mRNA in WT, Cnrtl^MO and Zip10^MO zebrafish embryos as measured by qPCR. Data are presented as the mean±S.E.M. (N=6 and the experiment was repeated with the same results). (C) Expression of Cdh1 protein during epipoly in WT and Zip10^MO embryos as assessed by immunohistochemistry (N=3) or (D) by immunoblots, which were (E) quantified by band intensity (mean±S.E.M., N=3). qPCR analysis of mRNA for the gastrulation-inducing gene products of (F) stat3 and (G) zip6. Data are expressed as log₂ fold change and error bars represent mean±S.E.M. (N=6 and the experiment was repeated with the same results). Data were statistically evaluated by ANOVA and significant differences (P<0.05) relative to the WT indicated by an asterisk.