FIGURE

Fig. S3

ID

ZDB-FIG-160901-17

Publication

Balastik et al., 2015 - Prolyl Isomerase Pin1 Regulates Axon Guidance by Stabilizing CRMP2A Selectively in Distal Axons.

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Fig. S3

Inhibition of Pin1 isomerase activity reduces axon growth and CRMP2A axon levels in primary cortical neurons. Overexpressed FLAG-tagged CRMP2A accumulates in distal axons. Related to Figure 4

Treatment of 1 DIV cortical neurons with Juglone, an inhibitor of Pin1 isomerase activity for 3 days (B) reduces CRMP2A levels in axons and axon growth as analyzed by CRMP2A and tau double immunostaining. (A) Untreated control. (C) Quantification of axon length. (p < 2e-06, means ± SEM values shown). (E-K) Expression of FLAG-tagged CRMP2A leads to CRMP2A accumulation in distal axons both in Pin1 WT (E) and KO (G) primary cortical neurons, as visualized by anti-FLAG antibodies. No FLAG signal was detected in vectortransfected Pin1 WT (D) or KO (F) neurons. (H-K) GFP signal used to trace the transfected neurons in (D-G). (Scale bars 100 µm), (D and H, E and I, G and K are composite images of 4, 7 and 6 images, respectively).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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