FIGURE

Fig. 7

ID

ZDB-FIG-160901-15

Publication

Balastik et al., 2015 - Prolyl Isomerase Pin1 Regulates Axon Guidance by Stabilizing CRMP2A Selectively in Distal Axons.

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7

Pin1 Regulates Sema3A Signaling In Vivo

(A and B) Efficiency of single- and double-morpholino KD of zPin1 and NRP1 in 24-hr zebrafish embryos analyzed by immunoblotting (A), with quantification in (B).

(C) Whole-mount immunostaining of acetylated tubulin in 1-day-old zebrafish embryos demonstrates increased incidence of developmental motor neurons defects, e.g., aberrant branching (arrow) or truncated growth (arrowhead), upon NRP1 KD, which is reduced upon co-injection of Pin1-MO.

(D) Quantification of developmental defects in morpholino-injected zebrafish embryos. Control embryos and Pin1-MO injected do not significantly differ in percentage of defective embryos (17% versus 27%, respectively) or average number of defects per embryo (0.22% versus 0.4%, respectively). NRP1 KD induces defective development in 85% of embryos, with an average of 2.05 defects per embryo. Co-injection of Pin1-MO significantly reduces number of defective embryos (44%) as well as the average number of defects per embryo (0.95).

(E-H) A model of Pin1’s role in Sema3A-driven axonal growth and retraction. Values are means ± SEM.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.