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ZFIN ID: ZDB-FIG-160831-10
Xu et al., 2016 - Microglia Colonization of Developing Zebrafish Midbrain Is Promoted by Apoptotic Neuron and Lysophosphatidylcholine. Developmental Cell   38(2):214-22 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Long-pec to Day 6
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Protruding-mouth to Day 6

Fig. 2

Microglia Colonization of the Optic Tectum Is Circulation Independent

(A) Coronal (top) and transverse (bottom) views of time-lapse imaging pictures of the midbrain of Tg(kdrl:eGFP;coro1a:DsRedx) zebrafish embryos reveal that microglia/microglial precursors (one microglia is marked by a magenta arrowhead) are outside vessels during their colonization of the optic tectum. Red signals represent coro1a-DsRedx+ microglia/microglial precursors. Green signals represent kdrl-GFP+ vessels within the ventral midbrain, whereas cyan signals indicate vessels above the ventral midbrain. The coronal views are stacked confocal images, whereas the transverse planes are single confocal images.

(B) Dorsal view of the optic tectum of Tg(-2.8elavl3:eGFP;coro1a:DsRedx) zebrafish embryos injected with or without the tnnt2a MO. The number of optic-tectum-resident microglia only slightly decreased in the 3 dpf tnnt2a morphants but is drastically reduced in the 6 dpf tnnt2a morphants. Red and green signals represent coro1a-DsRedx+ microglia and elavl3-GFP+ neurons, respectively. The optic tectum is indicated by dashed lines.

(C) Quantification of the number of optic-tectum-resident microglia in WT Tg(-2.8elavl3:eGFP;coro1a:DsRedx) embryos and tnnt2a morphants. Error bars represent the mean ± SEM. **p < 0.01, ***p < 0.001 (n = 6 for both WT and morphants at 3 dpf and 6 dpf).

(D) Measurement of the width of the optic tectum of WT Tg(-2.8elavl3:eGFP;coro1a:DsRedx) embryos and tnnt2a morphants. Error bars represent the mean ± SEM. ***p < 0.001 (n = 5 for 3 dpf WT, n = 6 for 6 dpf WT, n = 6 for morphants at both 3 dpf and 6 dpf).

(E) Coronal (top) and transverse (bottom) views of time-lapse imaging pictures of the midbrain of Tg(-2.8elavl3:eGFP;coro1a:DsRedx) zebrafish embryos show one microglia (labeled by white arrows) migrating out of the optic tectum in the 5.5 dpf tnnt2a morphants. The optic tectum is indicated by dashed lines. Red and green signals represent coro1a-DsRedx+ microglia and elavl3-GFP+ neurons, respectively. OT, optic tectum.

See also Figure S2 and Movie S2.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRedx hkz011tTg ; s843Tg standard conditions Long-pec to Protruding-mouth midbrain microglial cell IFL
hkz011tTg ; tsu11Tg standard conditions Protruding-mouth microglial cell IFL
Protruding-mouth optic tectum microglial cell IFL
Day 6 microglial cell IFL
Day 6 optic tectum microglial cell IFL
hkz011tTg ; tsu11Tg + MO1-tnnt2a standard conditions Protruding-mouth microglial cell IFL
Protruding-mouth optic tectum microglial cell IFL
Day 5 microglial cell IFL
Day 6 microglial cell IFL
Day 6 optic tectum microglial cell IFL
EGFP hkz011tTg ; s843Tg standard conditions Long-pec to Protruding-mouth midbrain blood vessel IFL
hkz011tTg ; tsu11Tg standard conditions Protruding-mouth brain neuron IFL
Protruding-mouth optic tectum neuron IFL
Day 6 brain neuron IFL
Day 6 optic tectum neuron IFL
hkz011tTg ; tsu11Tg + MO1-tnnt2a standard conditions Protruding-mouth brain neuron IFL
Protruding-mouth optic tectum neuron IFL
Day 5 optic tectum neuron IFL
Day 6 brain neuron IFL
Day 6 optic tectum neuron IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
hkz011tTg ; tsu11Tg + MO1-tnnt2a standard conditions Protruding-mouth heart contraction decreased occurrence, abnormal
Protruding-mouth optic tectum decreased width, abnormal
Protruding-mouth optic tectum microglial cell decreased amount, abnormal
Day 5 optic tectum microglial cell migration process quality, abnormal
Day 6 heart contraction decreased occurrence, abnormal
Day 6 optic tectum decreased width, abnormal
Day 6 optic tectum microglial cell decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 38(2), Xu, J., Wang, T., Wu, Y., Jin, W., Wen, Z., Microglia Colonization of Developing Zebrafish Midbrain Is Promoted by Apoptotic Neuron and Lysophosphatidylcholine, 214-22, Copyright (2016) with permission from Elsevier. Full text @ Dev. Cell