Fig. 7

Mn accumulates in the brain of slc39a14U801 mutants and can be lowered by Na2CaEDTA. Tissue Mn levels of wild-type (WT) and homozygous slc39a14^U801 zebrafish at 1 year of age show that mutant brain tissue accumulates significantly higher amounts of Mn (P=0.0012) while no differences are evident in the Mn content of abdominal viscera (P=0.116). Measurements were taken from pools of four brains/abdominal viscera. Data are presented as means±s.d. from two independent experiments. Statistical analysis was performed using Student’s two-tailed t-test (**P<0.01; NS, not significant). (b) Graph showing the effect of Na₂CaEDTA injections on Mn levels in homozygous slc39a14^U801 larvae. Mutant larvae exposed to 50 μM MnCl₂ from 2 to 5 dpf were injected into the heart with 5 or 50 ng of Na₂CaEDTA at 2, 3 and 4 dpf, resulting in a reduction of Mn levels to that of wild-type larvae (P=0.000 (WT untreated versus mutant untreated), P=0.001 (mutant untreated versus 5 ng), P=0.001 (mutant untreated versus 50 ng)). Measurements were taken from pools of 10 larvae. Data are presented as means±s.d. from a minimum of three independent experiments. Statistical analysis was performed using one-way ANOVA (P=0.000) and Tukey’s multiple comparison test (**P<0.01, ***P<0.001). ANOVA, analysis of variance.