FIGURE

Fig. 5

ID
ZDB-FIG-160705-4
Publication
Tuschl et al., 2016 - Mutations in SLC39A14 disrupt manganese homeostasis and cause childhood-onset parkinsonism-dystonia
Other Figures
All Figure Page
Back to All Figure Page
Fig. 5

Zebrafish slc39a14 is expressed during early zebrafish development and significantly reduced in slc39a14U801 mutants. (a) RT-PCR showing slc39a14 expression between 3 and 120 hpf in zebrafish. L, 100 bp ladder (Promega). (b) Whole-mount in situ hybridization using a DIG-labelled antisense RNA probe showing slc39a14 expression in the proximal convoluted (black arrows) and straight (red arrows) pronephric tubules in zebrafish larvae at 4 dpf. Top, lateral view; bottom, dorsal view. Scale bar, 200 µm. (c) DNA sequence of the region within exon 5 of slc39a14 targeted by a CRISPR guide RNA is highlighted in yellow and the 2-bp deletion introduced in the slc39a14U801 mutant indicated by dashes. Pam sequence underlined. (d) qRT-PCR demonstrates a 2.2-fold reduction in slc39a14 expression in homozygous slc39a14U801 mutants (*P=0.0117). Primers were designed to detect all slc39a14 transcripts (Supplementary Table 4). Ef1α was used as a reference gene. Data are presented as means±s.d. from three independent experiments. Statistical analysis was performed using Student’s two-tailed t-test on individual ΔCt values (*P<00.5).

Expression Data
Gene:
Fish:
Anatomical Terms:
Stage Range: 1k-cell to Day 5

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage: Day 4

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.