Fig. 3

Blood flow is required for Yap/Taz transcriptional activation in ORA and intramembranous ossification. (a) Stacked images of Tg(fli1: gal4-htead2ΔN): (UAS: GFP): (fli1: myr-mCherry) at the indicated hpf are shown. Green color represents Yap/Taz transcriptional activity. Red color represents all endothelial cells. The opercular artery (ORA), primary head sinus (PHS), common cardinal vein (CCV), and branchial arteries (AA) are also indicated. Average and SD of the total GFP intensity in ORA were measured from 20 embryos at each time-point and are shown in the right panel. Bar: 50 µm. (b) Similar experiments to (a) were performed with BDM treatment. Embryos without BDM treatment also shows as control. Tg(fli1: gal4-htead2ΔN): (UAS: GFP): (fli1: myr-mCherry) were treated with BDM from 54 hpf to the indicated hpf. Average and SD of the total GFP intensity in the ORA were measured from more than 18 embryos at each time-point. Bar: 50 µm. (c) Embryos were treated with or without BDM from 54 hpf to 72 hpf, stained with alizarin red s, and the volume of the opercle (op) and the diameter of the otolith (ot) were measured. Embryos without BDM treatment also shows as control. Average and SD were calculated from more than 10 embryos in each sample. Bar: 50 µm.