FIGURE

Fig. 4

ID

ZDB-FIG-160707-9

Publication

Davuluri et al., 2016 - Inactivation of 3-hydroxybutyrate dehydrogenase 2 delays zebrafish erythroid maturation by conferring premature mitophagy

Other Figures

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Fig. 4

Bdh2-inactivated erythrocytes contain mitochondria in autophagosomal vesicles. (A) Representative EM images of erythrocytes isolated from control and bdh2 morphants. Arrows indicate autophagosomal vesicles. Arrowheads indicate lysosomal vesicles. (Scale bars: 1 µM.) Area in rectangle is enlarged. (Scale bar: Inset, 100 nM.) (B) Total mitochondria count per cell counted by EM. Data are mean ± SD for 50 cells. P < 0.05 was considered significant. (C) Confocal fluorescent imaging of LysoTracker- and MitoTracker-stained erythrocytes isolated from control and bdh2 morphants or bdh2-overexpressing bdh2 morphants. Arrowheads indicate costained cells. Representative images are depicted.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-bdh2
Knockdown Reagent:	MO1-bdh2
Observed In:	erythroblast lysosome erythroblast mitochondrion erythroblast mitophagy
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA