FIGURE

Fig. 5

ID

ZDB-FIG-160630-28

Publication

Lovely et al., 2016 - Bmp signaling mediates endoderm pouch morphogenesis by regulating Fgf signaling in zebrafish

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Fig. 5

Blocking Bmp signaling does not alter endodermal cell number. (A-F′,M) Confocal images of sox17:dsred transgenic embryos labeled for cell death at 18 and 26hpf. Similar, low levels of apoptosis are present across all groups (18hpf, f=0, P=1; 26hpf, f=0.3, P=0.6, one-way ANOVA, n=4 embryos). (G-L′,N) Confocal images of sox17:dsred transgenic embryos labeled for cell proliferation at 18 and 26hpf. No differences in the level of proliferating endoderm cells are apparent (18hpf, f=0.2, P=0.8; 26hpf, f=0.1, P=0.8, one-way ANOVA, n=4 embryos). Arrows indicate overlap between the transgene and marker expression. Lateral views, anterior to the left. (O) Total endoderm cell numbers were counted via FACS and no statistically significant differences between 26hpf Dorsomorphin-treated, untreated or DMSO-treated embryos were observed (f=1.8, P=0.2, one-way ANOVA, n=8 samples, 10 embryos per sample). Error bars indicate mean+s.e.m. Scale bar: 50µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s903Tg
Condition:	chemical treatment: dorsomorphin
Observed In:	endodermal cell endodermal cell apoptotic process
Stage Range:	14-19 somites to Prim-5

Phenotype Detail

Acknowledgments

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