FIGURE

Fig. 8

ID

ZDB-FIG-160524-41

Publication

Hackett et al., 2016 - Chemical Biology in the Embryo: In Situ Imaging of Sulfur Biochemistry in Normal and Proteoglycan-Deficient Cartilage Matrix

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Fig. 8

FTIR spectroscopic imaging reveals a significant increase in total protein levels in cartilage from PG-deficient fam20b- mutant fish relative to wild-type fish. (A) FTIR spectroscopic images of total protein in wild-type and fam20b- mutant fish were generated from the integrated area under the amide I band (1600-1700 cm^-1). (B) Representative average FTIR spectra from the entire cartilage region of interest in one wild-type fish and one fam20b- mutant fish. (C) Average values calculated from the integrated area under the curve for the amide I band (1600-1700 cm^-1), the ester carbonyl band (1755-1715 cm^-1), and the amide I/ester carbonyl ratio. The integrated area under the curve values for the ester carbonyl band have been multiplied by a factor of 10 for the sake of clarity.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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