FIGURE

Fig. 2

ID

ZDB-FIG-160523-8

Publication

Abrams et al., 2016 - Graded effects of unregulated smooth muscle myosin on intestinal architecture, intestinal motility, and vascular function in zebrafish

Other Figures

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Fig. 2

Modifier mutations induce cell invasion in mlt heterozygotes. (A-D) Lateral views of live 6-dpf larvae. Arrowhead points to the mid-intestine. (A) A wild-type (WT) larva. (B) A homozygous mlt larva in which there is invasive expansion of the mid- and posterior intestine. (C) A mlt/S237Y compound heterozygote. Arrowhead points to a region of altered intestinal morphology. Inset shows a more severely affected mlt/S237Y compound heterozygote. (D) mlt/L1287M compound heterozygote. Mild and moderate (inset) phenotypes are shown as in C. (E,F) Histological section through the posterior intestine of 6-dpf mlt/S237Y and mlt/L1287M compound heterozygotes following their immunostaining with anti-Keratin (red) and anti-Laminin-1 antibodies (green). Dashed line indicates predicted position of basement membrane disrupted by the invasive epithelial cells; e, intestinal epithelium; sm, smooth muscle. (G,H) Histological section through the posterior intestine of 3-dpf Tg(miR194-Lifeact-GFP) wild-type and sibling mlt/S237Y larvae following immunostaining with anti-GFP (green) and anti-Laminin-1 (red) antibodies, showing GFP-labeled invadopodia (arrowheads) of epithelial cells extending through gaps in the basement membrane. Inset shows a higher-magnification view. (I-K) Confocal projections through the mid- and posterior intestine of 4-dpf Tg(miR194-Lifeact-GFP) wild-type and sibling mlt/S237Y (myh11^mlt/S237Y) larvae following anti-GFP immunostaining (green). Arrowheads point to invadopodia protruding from the basal epithelial cell plasma membrane in the mlt/S237Y mutants. Blue, DAPI-stained nuclei.

Expression Data

Gene:	GFP
Antibodies:	Ab1-lama1 Ab2-krt
Fish:	p306Tg myh11a^m498/+; myh11a^p318/+ myh11a^m498/+; myh11a^p318/+; p306Tg myh11a^m498/+; myh11a^p319/+
Anatomical Terms:	intestinal epithelium intestinal epithelium actin-based cell projection intestinal epithelium basement membrane
Stage Range:	Protruding-mouth to Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	myh11a^m498/+; myh11a^p318/+ myh11a^m498/+; myh11a^p318/+; p306Tg myh11a^m498/+; myh11a^p319/+
Observed In:	intestinal epithelium intestinal epithelium actin-based cell projection intestinal epithelium basement membrane intestine basement membrane mid intestine epithelial cell migration posterior intestine epithelial cell migration
Stage Range:	Protruding-mouth to Day 6

Phenotype Detail

Acknowledgments

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