FIGURE
Fig. 1
- ID
- ZDB-FIG-160513-10
- Publication
- Andrews et al., 2016 - Visualising apoptosis in live zebrafish using fluorescence lifetime imaging with optical projection tomography to map FRET biosensor activity in space and time
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Fig. 1
The Caspase 3 FRET biosensor design and its expression in transgenic fish. (A) In the Caspase 3 FRET biosensor, SECFP and Ypet are linked with a flexible 23 amino acid chain, which includes the Caspase 3 cleavage sequence DEVDG in the centre. Upon cleavage of this site in the presence of Caspase3, the SECFP and Ypet become unlinked and the fluorescence lifetime increases. (B) Tg(Ubi : Caspase3bios) zebrafish were generated using the TOL2 Gateway system. Founder fish were imaged at 5 dpf, using a GFP filter cube to assess transgene expression levels. |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
Acknowledgments
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Full text @ J. Biophotonics