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Fig. 1
The Caspase 3 FRET biosensor design and its expression in transgenic fish. (A) In the Caspase 3 FRET biosensor, SECFP and Ypet are linked with a flexible 23 amino acid chain, which includes the Caspase 3 cleavage sequence DEVDG in the centre. Upon cleavage of this site in the presence of Caspase3, the SECFP and Ypet become unlinked and the fluorescence lifetime increases. (B) Tg(Ubi : Caspase3bios) zebrafish were generated using the TOL2 Gateway system. Founder fish were imaged at 5 dpf, using a GFP filter cube to assess transgene expression levels.
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Full text @ J. Biophotonics