FIGURE

Fig. S3

ID

ZDB-FIG-160513-13

Publication

Andrews et al., 2016 - Visualising apoptosis in live zebrafish using fluorescence lifetime imaging with optical projection tomography to map FRET biosensor activity in space and time

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Fig. S3

A) Spectrally resolved fluorescence images of 48 hpf Tg(Ubi:Caspase3 biosensor) embryos: ?Donor channel? corresponds to donor excitation & donor channel detection; ?Acceptor channel? corresponds to acceptor excitation & acceptor channel detection; ?FRET channel? corresponds to donor excitation & acceptor channel detection and ?Corrected emission ratio? corresponds to the corrected mean acceptor/donor emission ratio calculated using FiJi following the protocol published by Kardash et al [27]. B) 48 hpf Tg(Ubi:SECFP) embryo (i.e. expressing only the donor fluorophore) showing cross-talk in acceptor channel. C) Graph showing a statistical significance of p>0.05 (*) between corrected mean acceptor/donor emission ratio of irradiated versus non-irradiated embryos. This corrected acceptor/donor emission ratio was averaged over all the pixels in each field of view and statistical analysis across multiple experiments was performed with n≥7 in each category using GraphPad Prism 6. Data showed a normal distribution so an unpaired t-test was used.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Biophotonics