FIGURE

Fig. 2

ID

ZDB-FIG-160512-13

Publication

Zhang et al., 2016 - Antiviral Drug Ganciclovir Is a Potent Inhibitor of the Proliferation of Müller Glia-Derived Progenitors During Zebrafish Retinal Regeneration

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Ganciclovir had no effect on Müller glia dedifferentiation and the initial MGPC formation. (A) Confocal microscopy of retinal sections after GFP and BrdU immunofluorescence shows the presence of GFP+ Müller glia near injury site in PBS- or GCV-treated retina at 4 dpi. Tg(1016tuba1a:GFP) fish were given a pulse of BrdU 3 hours before they were killed at 4 dpi. Note that there are many fewer BrdU+ MGPCs in GCV-treated retina than in control. Arrows indicate GFP+ columns. Each GFP+ column represents an activated Müller glia and its daughter progenitor cells. (B) Quantification of the number of GFP+ columns at the injury site at 4 dpi. #No significant difference, P > 0.05, n = 4. (C) Immunofluorescence shows the initial formation of MGPCs in the INL in PBS- or GCV-treated retina at 2 dpi. Tg(1016tuba1a:GFP) fish received a pulse of BrdU 3 hours before they were killed at 2 dpi. (D) Quantification of the number of BrdU+ MGPCs per injury at 2, 3, and 4 dpi in PBS- or GCV-treated retina. Fish received a pulse of BrdU 3 hours prior to killing at 2, 3, or 4 dpi. #No significant difference, P > 0.05; *P < 0.05; **P < 0.01. n = 4 for each group. Scale bars: 50 µm. The asterisks mark the injury site (needle poke).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Invest. Ophthalmol. Vis. Sci.