FIGURE

Fig. 1

ID

ZDB-FIG-160512-12

Publication

Zhang et al., 2016 - Antiviral Drug Ganciclovir Is a Potent Inhibitor of the Proliferation of Müller Glia-Derived Progenitors During Zebrafish Retinal Regeneration

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Fig. 1

Ganciclovir treatment significantly reduced the number of MGPCs after retinal injury. (A) Green fluorescent protein (green) and BrdU (red) immunofluorescence shows that GCV treatment decreased the number of BrdU+ MGPCs localized to the injury site at 4 dpi. DAPI (42,6-diamidino-2-phenylindole) channel (blue) was added to the overlay images to show retinal layer structures. Tg(1016tuba1a:GFP) fish received a pulse of BrdU 3 hours before they were killed at 4 dpi. (B) Quantification of BrdU+ MGPCs in (A). *P < 0.05; **P < 0.01 compared to PBS control, n = 4. (C) TUNEL staining and quantification of TUNEL+ cell numbers from PBS-, GCV-, or ouabain-treated retina at 3 dpi. Arrows mark TUNEL+ cells. #No significant difference, P > 0.05, n = 4. Scale bars: 100 µm. The asterisks mark the injury site (needle poke). ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer; dpi, days post injury; MGPCs, Müller glia-derived progenitor cells.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Invest. Ophthalmol. Vis. Sci.