FIGURE

Fig. 1

ID

ZDB-FIG-160420-2

Publication

Uribe et al., 2016 - A novel subset of enteric neurons revealed by ptf1a:GFP in the developing zebrafish enteric nervous system

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Fig. 1

The transgenic line Tg(ptf1a:GFP) marks a subset of neurons within the larval ENS. Maximum projection confocal stacks at (A-A′′) 4 dpf, (B-B′′) 5 dpf and (C-C′′) 6 dpf depicting ptf1a:GFP⁺ and Hu⁺ cells within the midgut (dashed lines), scale bar: 70 microns, for A-C. (D) Bar graph illustrating total number of ptf1a:GFP⁺ cells in the gut from 4 dpf to 6 dpf, error bars represent s.e.m., n=6. (E) Maximum intensity confocal projection along the z-axis reveals that ptf1a:GFP⁺/Hu⁺ cells co-localize in a concentric pattern along the gut tube at 6 dpf, scale bar: 100 microns. (F) Lateral view of a 6 dpf ptf1a:GFP⁺ larval fish. (G, H) Ventrolateral view following whole mount in situ hybridization against ptf1a reveals that ptf1a localizes to the brain, pancreas and midgut of 6 dpf larvae. (I) Bar graph illustrating the percentage of total Hu⁺ neurons that are ptf1a:GFP⁺ in the midgut at 5 dpf and 6 dpf, error bars represent s.e.m., n = 6. Int. bulb-intestinal bulb.

Expression Data

Genes:	EGFP ptf1a
Antibody:	Ab1-elavl
Fish:	WT jh1Tg
Anatomical Terms:	brain intestinal bulb enteric neuron mid intestine mid intestine enteric neuron pancreas
Stage Range:	Day 4 to Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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