PUBLICATION

A novel subset of enteric neurons revealed by ptf1a:GFP in the developing zebrafish enteric nervous system

Authors
Uribe, R.A., Gu, T., Bronner, M.E.
ID
ZDB-PUB-160213-17
Date
2016
Source
Genesis (New York, N.Y. : 2000)   54(3): 123-8 (Journal)
Registered Authors
Bronner-Fraser, Marianne, Uribe, Rosa
Keywords
enteric nervous system, ptf1a, serotonergic neuron, zebrafish
MeSH Terms
  • Recombinant Proteins/genetics
  • Recombinant Proteins/metabolism
  • Neurogenesis
  • Enteric Nervous System/cytology*
  • Enteric Nervous System/embryology
  • Enteric Nervous System/metabolism
  • Transcription Factors/genetics*
  • Transcription Factors/metabolism
  • Mice, Transgenic
  • Neural Crest/cytology*
  • Neural Crest/metabolism
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism*
  • Zebrafish/growth & development*
  • Cell Differentiation
  • Serotonin/metabolism
  • Animals
  • Neurons/metabolism*
(all 18)
PubMed
26865080 Full text @ Genesis
Abstract
The enteric nervous system, the largest division of the peripheral nervous system, is derived from vagal neural crest cells that invade and populate the entire length of the gut to form diverse neuronal subtypes. Here, we identify a novel population of neurons within the enteric nervous system of zebrafish larvae that express the transgenic marker ptf1a:GFP within the midgut. Genetic lineage analysis reveals that enteric ptf1a:GFP(+) cells are derived from the neural crest and that most ptf1a:GFP(+) neurons express the neurotransmitter 5HT, demonstrating that they are serotonergic. This transgenic line, Tg(ptf1a:GFP), provides a novel neuronal marker for a subpopulation of neurons within the enteric nervous system, and highlights the possibility that Ptf1a may act as an important transcription factor for enteric neuron development. This article is protected by copyright. All rights reserved.
Genes / Markers
Figures
Figure Gallery (3 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ba74TgTransgenic Insertion
    jh1TgTransgenic Insertion
      ne2466TgTransgenic Insertion
        1 - 3 of 3
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        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        No data available
        Fish
        Fish
        ba74Tg; jh1Tg; ne2466Tg
        jh1Tg
        WT
        1 - 3 of 3
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        Antibodies
        Orthology
        No data available
        Engineered Foreign Genes
        Marker Marker Type Name
        CreEFGCre
        DsRed2EFGDsRed2
        EGFPEFGEGFP
        GFPEFGGFP
        1 - 4 of 4
        Show
        Mapping
        No data available
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        Citation
        Uribe, R.A., Gu, T., Bronner, M.E. (2016) A novel subset of enteric neurons revealed by ptf1a:GFP in the developing zebrafish enteric nervous system. Genesis (New York, N.Y. : 2000). 54(3):123-8.