Fig. 3

etv5a is required for MCC development. (A) At 24 hpf, WISH analysis demonstrates etv5a loss of function via morpholino (MO) knockdown (etv5a MO, etv5a MO2) and deletion of the acidic domain (etv5a^Δacidic) resulted in reduced expression of the MCC markers odf3b and centrin 4 (cetn4) (purple) in the pronephros. Insets are a magnification of the MCC domain in both a lateral (top) and dorsal (bottom) view. (B) odf3b expression via WISH in 24 hpf embryos co-injected with etv5a capped RNA (cRNA) and each morpholino (MO) was not as reduced as MO injection alone. Injection of etv5a cRNA also did not produce a great change in odf3b expression in the pronephros. (C) Quantification demonstrates a significant decrease in average MCC number for cetn4 in all three etv5a knockdown versions. (D) etv5a loss of function (etv5a MO, etv5a MO2, and etv5a^acidic) produced a significant reduction in the average MCC number compared to the control, as marked by odf3b. Co-injection of etv5a cRNA and MO partially rescued the MCC phenotype seen in embryos injected with MO only, where injection of etv5a cRNA alone did not produce a significant change in average MCC number compared to the control. Images and quantification are representative of at least 50 embryos, and error bars denote standard error. p-values: *****p<0.001, ****p<0.002, **p<0.02.