FIGURE

Fig. 4

ID

ZDB-FIG-160401-3

Publication

Faure et al., 2016 - A workflow to process 3D+time microscopy images of developing organisms and reconstruct their cell lineage

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Fig. 4

Visualization and validation of the lineage tree reconstruction (zebrafish dataset Dr1).

Results from the zebrafish data set Dr1. All screenshots taken from the Mov-IT visualization interface, then tagged. (a) Cell division illustrated by three snapshots; time in hours post fertilization (h.p.f.) indicated top right; cell centres (cyan cubes) and cell paths (cyan lines) superimposed on two raw-data orthoslices showing the membranes (grey levels). (b) Flat representation of the cell lineage tree for three cell clones over 17 consecutive time steps: each cell is represented by a series of cyan squares, linked according to the cell’s clonal history; the cell dividing in Fig. 4a is circled. (c,d) Nucleus centre detection in a subpopulation of cells chosen at 11.61 h.p.f. Mov-IT visualization in ′checking mode′ adding short vertical white lines to the detected centres, and displaying correct nuclei in green and false positives in red; (c) all detected nuclei; (d) validated nuclei only.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.