FIGURE

Fig. 8

ID

ZDB-FIG-160324-7

Publication

Zhang et al., 2016 - An oxygen-insensitive Hif-3α isoform inhibits Wnt signaling by destabilizing the nuclear β-catenin complex

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Fig. 8

Genetic deletion but not knockdown of Hif-3α2 result in dynamic and compensatory changes in the expression of Wnt/β-catenin and BMP target genes.(A-C) Expression of boz (A), vent (B), and vox (C) in hif-3αΔ42 mutant embryos at the indicated stages. (D-F) Expression of boz (D), vent (E), and vox (F) in morphants at the indicated stages. (G,H) Expression of tp63 in hif-3αΔ42 mutant embryos (G) and morphants (H) at the indicated stages. In all above, the mRNA levels of the indicated genes were measure and presented as described above. Values are means +S.E. (n = 3). * and **p < 0.05 and P < 0.01. (I,J) Treatment of wild type and hif-3αΔ42 mutant embryos with LiCl (0.3 mM) and BIO (4 µM) results in no eyes or small eyes phenotypes. Representative views are shown in the left panel and quantification results are shown in the right panel. The total embryo number is shown on the top. P value of Chi analysis is shown.

Expression Data

Genes:	dharma tp63 vent vox
Fish:	WT hif1al^mi600/mi600 WT + MO3-hif1al
Knockdown Reagent:	MO3-hif1al
Anatomical Term:	whole organism
Stage Range:	Shield to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT hif1al^mi600/mi600 WT + MO3-hif1al
Conditions:	chemical treatment: 6-bromoindirubin-3'-oxime chemical treatment: lithium chloride
Knockdown Reagent:	MO3-hif1al
Observed In:	eye whole organism
Stage Range:	Shield to Prim-5

Phenotype Detail

Acknowledgments

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