Hif-3α2 knockdown leads to Wnt/β-catenin signaling phenotypes.(A) Wild type (WT), control MO (cMO), and Hif-3α2 targeting MO (Hif-3α2 MO)-injected embryos were raised to 24 hpf in normoxic water. They were lysed and subjected to Western blotting. (B) Wild-type embryos injected with Topflash plasmid DNA together with the indicated capped mRNA or MO were raised to 9 hpf and the luciferase activity was measured. Values are means + S.E. (n = 3). * and **p < 0.05 and 0.01. (C,D) Embryos injected with the indicated MOs were raised to 9 hpf and vent (C) and vox (D) mRNA levels were determined and presented as described above. (E) Phenotypes. The frequency of embryos with the indicated phenotypes is shown at the bottom of each panel. Scale bar = 200 µm. (F) hif-3αΔ42 mutant embryos are less sensitive to Hif-3α2 MO. Control MO or Hif-3α2 MO were injected into wild type (+/+) or F3 homozygous hif-3αΔ42 mutant embryos (-/-). The frequency of embryos with the abnormal phenotype (E) at 9 hpf was determined and shown. Total embryo number is shown on the top of each column. P value of Chi analysis is shown.
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