FIGURE

Fig. S13

ID

ZDB-FIG-160311-33

Publication

Guo et al., 2016 - Functional Investigation of a Non-coding Variant Associated with Adolescent Idiopathic Scoliosis in Zebrafish: Elevated Expression of the Ladybird Homeobox Gene Causes Body Axis Deformation

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Fig. S13

Severe convergent extension defects and body curvature in Tg(GATA2-1b:lbx1b) F1 embryos.

(A) The constructs used for transgenesis in zebrafish with the tol2 transposon system are shown. The cardiac specific promoter cmlc2 drives EGFP expression in the heart as a transgenic marker. (B) RT-PCR for 11 hpf embryos of Tg(GATA2-1b:MCS) F1 (MCS) and Tg(GATA2-1b:lbx1b) F1 (lbx1b) shows the elevated expression of lbx1b in lbx1b embryos. Ef-1α was used as a constitutive control. (C) Dorsal views of In situ hybridization for papc (paraxial mesoderm marker) at 11 hpf. Severe convergent defects were found in lbx1b embryos. The scale bar represents 200 µm. (D) Dorsal views of embryos at 48 hpf. Severe body curvature was observed in lbx1b embryos. The scale bar represents 1 mm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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