FIGURE

Fig. S9

ID

ZDB-FIG-160311-29

Publication

Guo et al., 2016 - Functional Investigation of a Non-coding Variant Associated with Adolescent Idiopathic Scoliosis in Zebrafish: Elevated Expression of the Ladybird Homeobox Gene Causes Body Axis Deformation

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Fig. S9

Effect of RhoA mRNA injection on body curvature of Tg(hsp:Gal-VP:EGFP:UAS:lbx1b) with heat shock at 12 hpf.

(A) Dorsal views of 48 hpf Tg(hsp:Gal-VP:EGFP:UAS:lbx1b) embryos upon heat shock (HS) at 12 hpf with buffer (HS12h) or RhoA mRNA (HS12h+RhoA) injection. (B) Quantitative analysis of body curvature in Tg(hsp:Gal-VP:EGFP:UAS:lbx1b) embryos upon HS at 12 hpf with buffer injection (lbx1b, n = 13) or RhoA mRNA injection (lbx1b+RhoA, n = 13). Significant change in axis development was not observed in RhoA-injected embryos with HS at 12 hpf. Severity of body curvature was quantified by the angle as shown in Fig 3E. F2 lines of driver and responder transgenic fish were used.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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