FIGURE

Fig. 2

ID

ZDB-FIG-160218-15

Publication

Jahangiri et al., 2016 - The AP-1 transcription factor component Fosl2 potentiates the rate of myocardial differentiation from the zebrafish second heart field

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Fig. 2

A ventricular cardiomyocyte deficit emerges in fosl2 mutant embryos after unperturbed linear heart tube morphogenesis. (A-C) Confocal images of fluorescent cardiomyocyte nuclei in hearts of 48hpf control sibling (CTRL; A; n=11), fosl2^fb15-/- (B; n=5) and fosl2^fb16-/- (C; n=5) Tg(cmlc2:DsRed2-nuc) embryos. (D) Bar graph showing mean total, atrial and ventricular cardiomyocyte numbers in each experimental group. Error bars represent s.d. **P<0.01, ****P<0.0001, n.s., not significant. (E,F) Confocal images of ventricular and outflow tract (OFT) regions of 52hpf control (E; n=10) and null mutant (F, n=4) embryos co-stained with antibodies recognizing striated muscle (MF20, red) or OFT smooth muscle (Elastin2, ELN2, green, arrow in E). (G-I) Confocal images of fluorescent cardiomyocyte nuclei in linear heart tubes of 24hpf control (G; n=12), fosl2^fb15-/- (H; n=5) and fosl2^fb16-/- (I; n=5) Tg(cmlc2:DsRed2-nuc) embryos. (J) Bar graph showing mean cardiomyocyte numbers in each experimental group at 24hpf. Error bars represent s.d. n.s., not significant. V, ventricle. A, atrium. Scale bars: 50µm.

Expression Data

Gene:	DsRed2
Antibodies:	Ab-MF20 Ab1-elnb
Fish:	WT fosl2^fb15/fb15 f2Tg fosl2^fb15/fb15; f2Tg fosl2^fb16/fb16; f2Tg
Anatomical Terms:	bulbus arteriosus smooth muscle cardiac muscle cell cardiac muscle cell nucleus
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fosl2^fb15/fb15 fosl2^fb15/fb15; f2Tg fosl2^fb16/fb16; f2Tg
Observed In:	atrium cardiac muscle cell bulbus arteriosus smooth muscle cardiac muscle cell cardiac ventricle cardiac muscle cell
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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