ZFIN ID: ZDB-FIG-160208-34
Shen et al., 2015 - Down-regulation of msrb3 and destruction of normal auditory system development through hair cell apoptosis in zebrafish. The International journal of developmental biology   59:195-203 Full text @ Int. J. Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Day 5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 5

Fig. 4

Down-regulation of msrb3. L1-8 neuromasts were decreased and disordered and hair cells were reduced. (A,B; D,E) Hair cells in lateral line neuromasts stained with FM-1-43FX. (A,B) In2MO and Ex2MO morphants had fewer and more disordered primary neuromasts than controls. Scale bars: 150 µm. (D,E) Msrb3 morphants had fewer hair cells per neuromast than controls. Scale bars: 5 µm. (C) Statistical analysis of the L1-8 neuromasts in mismatch controls and msrb3 morphants, “n” represents fish number. (F) Statistical analysis of hair cells per neuromast in mismatch controls and msrb3 morphants, “n” represents neuromasts number. Error bars are s.d. ***P<0.0001.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP s356tTg control Day 5 neuromast hair cell IFL
s356tTg + MO1-msrb3 control Day 5 neuromast hair cell IFL
s356tTg + MO2-msrb3 control Day 5 neuromast hair cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s356tTg + MO1-msrb3 control Day 5 neuromast decreased amount, abnormal
Day 5 neuromast hair cell decreased amount, abnormal
s356tTg + MO2-msrb3 control Day 5 neuromast decreased amount, abnormal
Day 5 neuromast hair cell decreased amount, abnormal
Acknowledgments:
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