ZFIN Figure: Marsden et al., 2015, Fig. 1

Fig. 1

The Mauthner Cell Is Active Only during Short-Latency C-Bends and Not during Long-Latency C-Bends

(A) The larval zebrafish acoustic startle circuit: hair cells in the otic vesicle (purple), VIII nerve afferents (green), hindbrain Mauthner cells (M-cells, blue), spinal motor neurons (pink), and contraction of muscle (brown).

(B) High-speed images (500 Hz) of a short-latency C-bend (SLC) in a head-restrained 5-dpf larva. Arrowheads highlight tail (scale bar, 1 mm).

(C) Distribution of startle latencies in head-restrained larvae (NR, no response; LLC, long-latency C-bend).

(D) Representative example of M-cell activation following acoustic stimulation in a Gal4FF62a;UAS:GCaMP6s transgenic larva. Arrowhead highlights lateral dendrite activation. Color scale denotes fluorescence intensity (black, lowest; white, highest; scale bar, 10 �m).

(E) Averaged traces show change in fluorescence (ΔF) relative to baseline (F₀) for SLC (red) and LLC (blue) responses performed to the contralateral side of the imaged M-cell. Gray lines indicate SEM.

(F) Scatterplot of peak ΔF/F₀ values for contralateral SLCs, LLCs, and NRs. (p < 0.0001, t test). Error bars indicate SEM.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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