Fig. 4

Amiodarone-induced zebrafish s-vcanb can repress EGFR signaling.

WISH of snail1b (A, B) and cdh5 (C, D) in control (A, C) and AG1478 (B, D)-treated embryos. When AG1478 inhibited EGFR activity, snail1b (B) in the AVC was lost, but cdh5 (D) was greatly induced. The number of embryos displaying a similar pattern was indicated. (E) EGFR-targeting antibodies were used to depict EGFR monomers and dimers in 72 hpf WT and Amiodarone-treated embryos. (F) Amiodarone can repress the phosphorylation of EGFR on Tyr-845. (G) Knockdown of s-vcanb (vMO) can rescue the reduction of EGFR phosphorylation on Tyr-845 in Amiodarone-treated embryos, but knockdown of vcana (aMO) cannot. (H) Although Gsk3b activity was increased in Amiodarone-treated embryos, it could be rescued by knockdown of s-vcanb, but not vcana. 15A: 15µM Amiodarone treatment; 30A: 30 µM Amiodarone treatment. The relative intensities of each protein were as indicated. The α-tubulin was used as an internal control.