FIGURE

Fig. 2

ID

ZDB-FIG-160205-48

Publication

Cronan et al., 2015 - CLARITY and PACT-based imaging of adult zebrafish and mouse for whole-animal analysis of infections

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Fig. 2

PACT protocol maintains integrity of blood vessels. (A-D) Blood vessels in gills labeled by Tg(flk1:egfp) in a zebrafish adult pre-clearing (immediately post-euthanasia) and the same animal (E-H) post-clearing. Increasing numbers of gill blood vessels are visible deeper within the body following clearing (G compared to C). Single Z frames (D,H, and insets) demonstrate that fine structures are unaffected by the clearing process. (I,J) Blood vessels in the tail of the same animal pre- and post-clearing. (K,L) Large blood vessels in the mid-trunk of the same animal pre- and post-clearing. Scale bars: 120µm. Single Z frames were exported and gamma adjusted in FIJI/ImageJ for increased visibility, with all gamma adjustments applied uniformly across all images.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.