FIGURE

Fig. 4

ID

ZDB-FIG-160205-50

Publication

Cronan et al., 2015 - CLARITY and PACT-based imaging of adult zebrafish and mouse for whole-animal analysis of infections

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Fig. 4

Fluorescent mycobacteria and cytokine induction can be imaged deep within intact adult zebrafish. (A) The TNF reporter (green) is expressed throughout a large granuloma (tdTomato-expressing M. marinum: magenta) in the TgBAC(tnf:GFP) line. Imaging begins 256µm below the scales and the stack (A) is µ100µm deep; individual Z planes from the stack (B-D) reveal TNF reporter intensity throughout the granuloma. (E-H) TNF reporter expression in the granuloma is not dependent on infection status of individual cells: (F) an infected cell that does not express the TNF reporter; (G) an infected cell expressing the TNF reporter; (H) an uninfected cell expressing the TNF reporter. Scale bars: 50µm (A-E); 5µm (F-H). Single Z frames were exported and gamma adjusted in FIJI/ImageJ for increased visibility, with all gamma adjustments applied uniformly across all images.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.