ZFIN Image: Cronan et al., 2015, Fig. 2

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Figure Caption

Fig. 2

PACT protocol maintains integrity of blood vessels. (A-D) Blood vessels in gills labeled by Tg(flk1:egfp) in a zebrafish adult pre-clearing (immediately post-euthanasia) and the same animal (E-H) post-clearing. Increasing numbers of gill blood vessels are visible deeper within the body following clearing (G compared to C). Single Z frames (D,H, and insets) demonstrate that fine structures are unaffected by the clearing process. (I,J) Blood vessels in the tail of the same animal pre- and post-clearing. (K,L) Large blood vessels in the mid-trunk of the same animal pre- and post-clearing. Scale bars: 120µm. Single Z frames were exported and gamma adjusted in FIJI/ImageJ for increased visibility, with all gamma adjustments applied uniformly across all images.

Acknowledgments

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