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ZIRC
ZFIN ID: ZDB-FIG-151214-56
Takeuchi et al., 2015 - LSD1/KDM1A promotes hematopoietic commitment of hemangioblasts through downregulation of Etv2. Proc. Natl. Acad. Sci. USA   112(45):13922-7 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage Range: 2-cell to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage Range: 1-4 somites to Days 7-13

Fig. S2

Nonhematopoietic defects in lsd1it627 embryos. (A) Expression of lsd1 at the indicated developmental stages was analyzed in WT or lsd1it627 embryos. Ubiquitous expression of lsd1 in zebrafish embryos was impaired in lsd1it627 mutants. (B) Survival rates of lsd1it627 mutants calculated using the Kaplan–Meier method. (C) Results of a comparison of the phenotypes between lsd1it627 and gata1m651 mutants at 4 dpf. The arrows and arrowheads indicate the swim bladder and erythrocytes in the heart, respectively. The presence of pale erythrocytes due to erythropoietic defects was detected in both lsd1it627 and gata1m651 mutants, whereas defects in the swim bladder were only observed in lsd1it627 mutants.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
kdm1a AB/TL standard conditions 2-cell whole organism ISH
Sphere whole organism ISH
1-4 somites whole organism ISH
5-9 somites whole organism ISH
20-25 somites whole organism ISH
Prim-5 whole organism ISH
kdrl kdm1ait627/it627(AB/TL) standard conditions 1-4 somites Not Detected whole organism ISH
Prim-5 Not Detected whole organism ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gata1am651/m651 standard conditions Day 4 erythrocyte differentiation decreased process quality, abnormal
kdm1ait627/it627(AB/TL) standard conditions 1-4 somites whole organism kdrl expression absent, abnormal
kdm1ait627/it627(AB/TL) standard conditions Prim-5 whole organism kdrl expression absent, abnormal
kdm1ait627/it627(AB/TL) standard conditions Day 4 erythrocyte differentiation decreased process quality, abnormal
kdm1ait627/it627(AB/TL) standard conditions Day 4 swim bladder inflation decreased process quality, abnormal
kdm1ait627/it627(AB/TL) standard conditions Days 7-13 whole organism decreased life span, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proc. Natl. Acad. Sci. USA for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA